Concept

Biotinylation

Publications associées (12)

Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics

Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz

The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...

ROYAL SOC CHEMISTRY2023

A molecular logic gate enables super-resolved imaging of intracellular lipid droplets

Pablo Rivera Fuentes, Adam Miklos Eördögh

Photoactivatable dyes enable single-mol. imaging in biol. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, li ...

2019

High-Performance Protein Preconcentrator Using Microchannel-Integrated Nafion Strip

Martinus Gijs, Venkataragavalu Sivagnanam, Hui Yang, Meng Shen

A microfluidic device for protein preconcentration based on the electrokinetic trapping principle is proposed. It comprises a Nafion strip which is simply cut from a commercially available membrane and integrated into a polydimethylsiloxane (PDMS) microflu ...

IEEE2011

Dynamic Actuation of Magnetic Beads for Immunoassays on-chip

Yves Moser

This thesis was carried out in the frame of the European project DetectHIV aiming the development of a new biosensor platform for the highly sensitive detection of the HIV capsid protein p24. We explore the implementation of a magnetic bead-based lab-on-a- ...

EPFL2010

Capacitance DNA bio-chips improved by new probe immobilization strategies

Giovanni De Micheli, Sandro Carrara, Yusuf Leblebici, Luca Benini, Andrea Cavallini, Bruno Samori

Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is apromising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation,scatt ...

2010

Nonfouling Polypeptide Brushes via Surface-initiated Polymerization of N-epsilon-oligo(ethylene glycol)succinate-L-lysine N-carboxyanhydride

Jian Wang, Harm-Anton Klok, Matthew Gibson, Raphaël Barbey

This contribution describes the preparation of nonfouling polypeptide brushes via surface-initiated ring-opening polymerization of oligo(ethylene glycol) modified L-lysine N-carboxyanhydrides. Circular dichroism experiments indicated that these surface-anc ...

2009

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Theo Lasser, Kai Hassler

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines ...

2007

An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells

Oliver Hantschel

Tandem affinity purification (TAP) is a generic two-step affinity purification protocol that enables the isolation of protein complexes under close-to-physiological conditions for subsequent analysis by mass spectrometry. Although TAP was instrumental in e ...

Nature Publishing Group2006

Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts

Philipp Bucher

The function of DNA-binding proteins is controlled not just by their abundance, but mainly at the level of their activity in terms of their interactions with DNA and protein targets. Moreover, the affinity of such transcription factors to their target sequ ...

2005

Bioconjugates for bioseparation by affinity precipitation

Matteo Costioli

Recent advances in the fields of biotechnology and medicine have intensified the necessity for pharmaceutical-grade biomacromolecules purification (e.g. enzymes, antibodies, plasmid DNA). One of the key factors for a successful downstream process is the po ...

EPFL2004

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