Michael Stefan Daniel Liebling

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Publications associées (19)

Reconstruction of image sequences from ungated and scanning-aberrated laser scanning microscopy images of the beating heart

Michael Stefan Daniel Liebling, Olivia Mariani

Fluorescence laser-scanning microscopy is a well-established imaging technique in biology, available in many imaging facilities to investigate structures within live animal embryos such as zebrafish. Laser scanning microscopes (LSM) are limited when used t ...

2020

Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

Semi-Blind Spatially-Variant Deconvolution in Optical Microscopy with Local Point Spread Function Estimation By Use Of Convolutional Neural Networks

Michael Stefan Daniel Liebling, Adrian Shajkofci

We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy algorithm. To f ...

2018

Direct inversion algorithm for focal plane scanning optical projection tomography

Michael Stefan Daniel Liebling

2017

Simultaneous temporal superresolution and denoising for cardiac fluorescence microscopy

Michael Stefan Daniel Liebling

Due to low light emission of fluorescent samples, live fluorescence microscopy imposes a tradeoff between spatiotemporal resolution and signal-to-noise ratio. This can result in images and videos containing motion blur or Poisson-type shot noise, depending ...

2016

A Point-Spread-Function-Aware Filtered Backprojection Algorithm for Focal-Plane-Scanning Optical Projection Tomography

Michael Stefan Daniel Liebling

Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in bl ...

2016

Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration

Michael Stefan Daniel Liebling

We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet micros ...

2015

Comparing Algorithms for Reconstructing Digital Off-Axis Fresnel Holograms

Michaël Unser, Michael Stefan Daniel Liebling

Three-dimensional information about an object, such as its depth, may be captured and stored digitally in a single, two-dimensional, real-valued hologram acquired in an off-axis geometry. Digital reconstruction of the hologram permits the quantitative retr ...

SPIE2005

Continuous Wavelet Transform Ridge Extraction for Spectral Interferometry Imaging

Michaël Unser, Michael Stefan Daniel Liebling, Theo Lasser

The combination of wavelength multiplexing and spectral interferometry allows for the encoding of multidimensional information and its transmission over a mono-dimensional channel; for example, measurements of a surface's topography acquired through a mono ...

SPIE2005

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