Jean-Manuel Segura

Cette personne n’est plus à l’EPFL

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Publications associées (14)

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Horst Vogel, Kai Johnsson, Rudolf Hovius, Jean-Manuel Segura, Nathalie George, Pedro Pascoal, Bruno Meyer

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

2006

CD8+ Cytotoxic T Lymphocyte Activation by Soluble Major Histocompatibility Complex-Peptide Dimers

Horst Vogel, Jean-Manuel Segura, Olivier Michielin, Bruno Meyer

CD8+ cytotoxic T lymphocyte (CTL) can recognize and kill target cells that express only a few cognate major histocompatibility complex class I-peptide (pMHC) complexes. To better understand the mol. basis of this sensitive recognition process, the authors ...

2005

Synthesis of nanoscopic optical fibers using lipid membranes as templates

Horst Vogel, Rudolf Hovius, Kurt Schenk, Pierre Stadelmann, Jean-Manuel Segura, Marinela Gjoni Boillat, Gopakumar Gopalakrishnan, Dimitrios Stamou, Cédric Gaillard

High-aspect-ratio, monocryst. Cd chloride nanowires doped with fluorescent CdS nanoparticles were synthesized using planar lipid membranes as templates. Cd2+ ions bound to the lipid bilayers (a) are released at low pH to the nanometer-sized, interlamellar ...

Wiley-VCH Verlag GmbH2005

Reversible sequential-binding probe receptor-ligand interactions in single cells

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005

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