Publications de Luigino Grasso

The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles

Horst Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Luigino Grasso, Ghérici Hassaïne

The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodie ...

Elsevier2016

Use of fourier transform infrared spectroscopy analysis of extracellular vesicles isolated from body fluids for diagnosing, prognosing and monitoring pathophysiological states and method therfor

Horst Vogel, Luigino Grasso, Romain Wyss

The method analyzes biological fluids collected from patients, and uses attenuated total reflecting (ATR) sensors and spectral analysis by Fourier transform infrared spectroscopy (FTIR) to form a spectral fingerprint of extracellular vesicles (EVs) isolate ...

2016

Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy

Horst Vogel, Niels Erik Lion, Davide Demurtas, Luigino Grasso, Romain Wyss, Michel Prudent, Ashwin Thampi, Lorenz Weidenauer

We report on a generic method to detect and iden- tify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identit ...

Springer Heidelberg2015

X-ray structure of the mouse serotonin 5-HT3 receptor

Horst Vogel, Henning Paul-Julius Stahlberg, Rudolf Hovius, Cédric Deluz, Luigino Grasso, Romain Wyss, Menno Berend Tol, Ghérici Hassaïne

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

Nature Publishing Group2014

Molecular and Dimensional Profiling of Highly Purified Extracellular Vesicles by Fluorescence Fluctuation Spectroscopy

Horst Vogel, Luigino Grasso, Davide Demurtas, Romain Wyss, Hans Wolfgang Grosse, Camille Wolf

Cells secrete extracellular vesicles (EVs) into their microenvironment that act as mediators of intercellulard communication under physiological conditions and in this context also actively participate in spreading various diseases. Large efforts are curre ...

Amer Chemical Soc2014

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Horst Vogel, Rudolf Hovius, Luigino Grasso, Romain Wyss, Ghérici Hassaïne, Joachim Piguet, Michael Werner

Cellular signaling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show that ligand binding to cell surface receptors and subsequent activation of signaling cascades can be monitor ...

Public Library of Science2013

Transmembrane Signaling Analysis in Model Membrane Systems

Luigino Grasso

Studying the complex mechanisms of transducing an extracellular signal across a plasma membrane to initiate an intracellular responce is of fundamental importance for a cell’s function. Transmembrane signaling is the key allowing cells to sense and communi ...

EPFL2012

Recombinant expression and functional characterization of the mouse olfactory receptor mOR256-17 in mammalian cells

Horst Vogel, Horst Pick, Luigino Grasso, Thamani Dahoun

Olfactory receptors (ORs) constitute the largest family of sensory membrane proteins in mammals. They play a key role within the olfactory system in recognizing and discriminating a nearly unlimited number of structurally diverse odorous molecules. The mol ...

2011

Protein-Binding Microarray Analysis of Tumor Suppressor AP2 alpha Target Gene Specificity

Horst Vogel, Luigino Grasso, Tanja Egener-Kuhn, Nicolas Mermod, Jan Kerschgens, Hans-Anton Lehr

Cheap and massively parallel methods to assess the DNA-binding specificity of transcription factors are actively sought, given their prominent regulatory role in cellular processes and diseases. Here we evaluated the use of protein-binding microarrays (PBM ...

Public Library of Science2011

Deamidation and Transamidation of Substance P by Tissue Transglutaminase Revealed by Electron-Capture Dissociation Fourier Transform Mass Spectrometry (Chem. Eur. J. 2/2011)

Horst Vogel, Yury Tsybin, Luigino Grasso, Adrian Schmid, Luca Fornelli

Tissue transglutaminase (tTGase) catalyzes both deamidation and transamidation of peptides and proteins by using a peptidyl glutamine as primary substrate. A precise consensus sequence for the enzyme is unknown and the ratio between deamidated and transami ...

Wiley-Blackwell2011