Êtes-vous un étudiant de l'EPFL à la recherche d'un projet de semestre?
Travaillez avec nous sur des projets en science des données et en visualisation, et déployez votre projet sous forme d'application sur GraphSearch.
We propose an original concept to perform a complete on-chip sandwich immunoassay on magnetic nanoparticles that are self-assembled in chains in a uniform magnetic field. The magnetic chains are retained over periodically enlarged cross sections of a microfluidic channel. Thereby they strongly interact with the flow and rapidly capture the total of a low number of target molecules from nanoliter sample volumes. As an example, we demonstrate the detection of murine monoclonal antibodies in a noncompetitive sandwich immunoassay with a detection limit of 1 ng mL-1 in nanoliters of hybridoma cell culture medium.
Chargement
Chargement
Chargement
Chargement
Martinus Gijs, Frédéric Lacharme, Caroline Vandevyver
Hatice Altug, Alexander Belushkin, Xiaokang Li, Maria Soler Aznar, Filiz Yesilköy