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Cell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysio ...
This thesis explores transport phenomena in nanochannels on a chip. Fundamental nanofluidic ionic studies form the basis for novel separation and preconcentration applications for proteomic purposes. The measurements were performed with 50-nm-high 1D nanoc ...
We report the detection of protein molecules with nanofabricated pores using the resistive pulse sensing method. A 20-nm -thick silicon nitride membrane with a nanofabricated pore measuring about 55 nm in diameter separated an electrolyte cell into two com ...
G protein-coupled receptors (GPCR) constitute by far the largest family of transmembrane cell-surface proteins involved in signal transduction and are the most important targets for the development of novel therapeutic compounds. Many processes mediated by ...
In nanometer-sized apertures with charged surfaces, the extension of the electrical double layer results in the electrostatic exclusion of co-ions and enrichment in counterions, which affects the permselectivity of such structures. A modeling of this pheno ...
In this thesis, two different fluorescent labeling techniques for in vivo investigations on the 5-HT3 receptor (5-HT3R) functions are presented. This plasma membrane protein contains five subunits surrounding an ion channel that opens after binding of a 5- ...
Interaction forces between ionizable surfaces across an electrolyte solution on the Poisson-Boltzmann level are discussed within the constant regulation approximation. The chemical response of each surface is expressed in terms of two parameters, namely, t ...
The outer membrane protein OmpF from Escherichia coli is a member of a large family of b-barrel membrane proteins. Some, like OmpF, are pore-forming proteins while others are active transporters or enzymes. We have previously shown that the receptor-bindin ...
Comprehensive counterion activity studies have revealed clear influence of the polyelectrolyte concn. and chain length on the polyion-counterion interaction, neither of which is evident from the existing theor. models. The counterion activity coeff. (fa) i ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...