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Optimization of microfluidic single cell trapping for long-term on-chip culture

Publications associées (55)

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Division site selection linked to inherited cell surface wave troughs in mycobacteria

Georg Fantner, John McKinney, Neeraj Dhar, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Haig Alexander Eskandarian, Mélanie Thérèse Marie Hannebelle, Joëlle Xiao Yuan Ven

Cell division is tightly controlled in space and time to maintain cell size and ploidy within narrow bounds. In bacteria, the canonical Minicell (Min) and nucleoid occlusion (Noc) systems together ensure that division is restricted to midcell after complet ...

2017

Development of microfluidic devices for chemotaxis of primary stem cells

Zuzana Tatárová

Functional analysis of primary tissue-specific stem cells is hampered by their rarity. Here I developed greatly miniaturized microfluidic devices for the multiplexed, quantitative analysis of the chemotactic properties of primary, bone marrow-derived mesen ...

EPFL2016

Microfluidic co-culture platform to quantify chemotaxis of primary stem cells

Zuzana Tatárová

Functional analysis of primary tissue-specific stem cells is hampered by their rarity. Here we describe a greatly miniaturized microfluidic device for the multiplexed, quantitative analysis of the chemotactic properties of primary, bone marrow-derived mese ...

Royal Soc Chemistry2016

Microwell and impedance-based approaches to isolate single cells for life sciences

Georges Muller

Cellular heterogeneity is ubiquitous in any cell population. Understanding these variations is key to answering fundamental questions in immunology, developmental biology, cancer or stem cell biology. For instance, single-cell in vitro and in vivo assays h ...

EPFL2016

Capturing epidermal stemness

Andrea Zaffalon

Regenerative medicine aims to replace or regenerate tissues or organs to re-establish their normal function. In 1975, Rheinwald and Green developed a technique to isolate and amplify epidermal stem cells. Their discovery led to the development of cultured ...

EPFL2016

Mechanical and Functional Study of Cell Physiology at the Nanoscale

Pascal Damian Odermatt

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016

Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells

Gioele La Manno, Jesper Ryge

Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA s ...

2016

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The impact of cellular individuality on host-microbe interactions is increasingly appreciated but studying the temporal dynamics of single-cell behavior in this context remains technically challenging. Here we present a microfluidic platform, InfectChip, t ...

Royal Society of Chemistry2016

Stem cells play a key role in a wide range of biological processes, in large part due to their ability to self-renew or differentiate into specialized cell types in response to various biological cues. In vivo, stem cells reside in a complex microenvironme ...

EPFL2015

Stem cell niche engineering through droplet microfluidics

Matthias Lütolf, Simone Allazetta

Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical ...

Current Biology Ltd2015