We present an active mixer based on the creation of virtual source-sink pairs in a polydimethylsiloxane (PDMS) chip. Perfect mixing is achieved in only a sub-second time interval for a compact 5 μL mixing volume. This excellent performance is achieved thanks to the implementation of co-rotating vortices inside the mixing chamber by using micro-plumbing technology. A DNA isolation and purification protocol using magnetic beads in the mixing chamber is per-formed on-chip, showing the enhanced DNA binding performance due to active mixing.
Edoardo Charbon, Francesco Piro, Ashish Sharma
David Atienza Alonso, Marina Zapater Sancho, Giovanni Ansaloni, Alexandre Sébastien Julien Levisse, Halima Najibi
Ileana-Cristina Benea-Chelmus, Jérôme Faist, Francesca Fabiana Settembrini