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Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Publications associées (51)

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High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics

Christoph Merten

During the last 25 years, display techniques such as phage display have become very powerful tools for protein engineering, especially for the selection of monoclonal antibodies. However, while this method is extremely efficient for affinity-based selectio ...

2010

Rationalization of the inhibition activity of structurally related organometallic compounds against the drug target cathepsin B by DFT

Paul Joseph Dyson, Angela Casini, Fabio Edafe

A series of organometallic compds. of general formula [(arene)M(PTA)nXm]Y (arene = η6-C10H14, η-C5Me5); (M = Ru(II), Os(II), Rh(III) and Ir(III); X = Cl, mPTA; Y = OTf, PF6) have been screened for their cytotoxicity and ability to inhibit cathepsin B in vi ...

Royal Society of Chemistry2010

Smart SU-8 Pillars Implemented in a Microfluidic Bioreactor for Continuous Measurement of Glucose

Nico de Rooij, Peter Van der Wal, Olivier Frey, Sara Talaei, Sotiria Psoma

In this contribution we explore a new and simple approach for immobilizing enzymes like glucose oxidase on SU-8 surfaces to develop a smart substrate integrated in microfluidics. SU-8 is a well known photoresist often used in microfluidic prototyping. Immo ...

Elsevier Science, Reg Sales Off, Customer Support Dept, 655 Ave Of The Americas, New York, Ny 10010 Usa2010

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Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a mo ...

American Chemical Society2009

Homing endonucleases (HE) are double-stranded DNAses that target large recognition sites (12-40 bp). HE-encoding sequences are usually embedded in either introns or inteins. Their recognition sites are extremely rare, with none or only a few of these sites ...

Springer Verlag2009

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This paper describes the development of a new method to obtain aqueous-core microcapsules from organic-core capsules. The direct production of microcapsules, using tripropionin as organic material, followed by the hydrolysis of the core by a lipase was inv ...

2008

Integrated nanoreactor system

Pierre-Yves Bolinger

This thesis describes a method that allows the production in parallel of nano-reactor systems whose function is controlled by a remote stimulus. The reactors comprise a nested system of lipid vesicles part of which release their content during a thermotrop ...

EPFL2006

Computational discovery of biochemical routes to specialty chemicals

Vassily Hatzimanikatis

A computational framework has been developed for the construction and evaluation of metabolic pathways given input substrates and knowledge of enzyme-catalyzed reactions. Application of the framework creates new and existing routes to both chems. known to ...

2004

Engineering a thermostable human prolyl endopeptidase for antibody-directed enzyme prodrug therapy

Christian Heinis, Dario Neri

We present a new antibody-directed enzyme prodrug therapy strategy (ADEPT) based on a post-proline cleaving endopeptidase and prodrugs, in which cytotoxic moieties are linked to a proline-contg. peptide. Human prolyl endopeptidase was expressed in Escheric ...

2004

Labeling of fusion proteins by enzymic incorporation of a coenzyme A derivative into an acyl carrier protein moiety

Kai Johnsson, Nathalie George

A method for labeling acyl carrier protein (ACP) fusion proteins using derivs. of CoA is described. The method relies on the transfer of a label from a CoA type substrate to an ACP fusion protein using a holo-acyl carrier protein synthase (ACPS) or a homol ...

(EPFL-Ecole Polytechnique Federale de Lausanne, Switz.).2004