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Frequent and accurate concentration estimates are important for the on-line control and optimization of chemical reaction systems. Such estimates can be obtained using state estimation methods that fuse frequent (fast) delay-free on-line measurements with infrequent (slow) delayed laboratory measurements. In this paper, we demonstrate how several recent advances made in state estimation can be combined in an on-line recursive state estimation framework by imposing knowledge-based and measurement-based constraints on the state estimates of multi-rate concentration measurements with time-varying time delays. This framework is illustrated using a simulated example for a bacterial batch fermentation of recombinant l. lactis. It is shown that an extent-based formulation gives more accurate estimates than a conventional concentrationbased formulation.
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