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In this thesis a new scanning near field optical microscope based on an apertureless scattering technique is introduced for resolving optical properties of surfaces with lateral resolution reaching 10 nm and better. The construction of the instrument is ba ...
In this thesis, we describe new approaches and methods for reconstructing complex-valued wave fields from digital holograms. We focus on Fresnel holograms recorded in an off-axis geometry, for which operational real-time acquisition setups readily exist. T ...
We present a new digital two-step reconstruction method for off-axis holograms recorded on a CCD camera. First, we retrieve the complex object wave in the acquisition plane from the hologram's samples. In a second step, if required, we propagate the wave f ...
In this paper we present recent single molecule detection experiment using a solid immersion lens (SIL) for fluorescent correlation spectroscopy measurements. We compared the performance of the SIL in combination with an air objective (40×, numerical apert ...
Optical near field microscope for use with different, including differential contrast, methods. Microscope has a near field illuminating light source probe (3) and a photo-detector for detection of light from the object (6). Said detector has electronics f ...
There have been three basic approaches to optical tomography since the early 1980s: diffraction tomography, diffuse optical tomography and optical coherence tomography (OCT). Optical techniques are of particular importance in the medical field, because the ...
Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude p ...
A volume hologram has two degenerate Bragg-phase-matching dimensions and provides the capability of volume holographic imaging. We demonstrate two volume holographic imaging architectures and investigate their imaging resolution, aberration, and sensitivit ...
An intelligent pinhole for confocal microscopy consisting of four position controlled blades forming a rectangular aperture from 3x3μm to 500 x 500 μm was built. This aperture can be positioned without drift under computer control in a positioning range wi ...
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissue ...
