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Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Publications associées (43)

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Temporal Super-Resolution Microscopy Using a Hue-Encoded Shutter

Sylvain Calinon, Michael Stefan Daniel Liebling, Emmanuel Pignat, Christian Jaques

Limited time-resolution in microscopy is an obstacle to many biological studies. Despite recent advances in hardware, digital cameras have limited operation modes that constrain frame-rate, integration time, and color sensing patterns. In this paper, we pr ...

2019

Biomedical optical techniques for intracochlear cellular imaging

Marilisa Romito

Imaging the inner ear microanatomy is of great importance for the assessment of the state of the cells responsible for sound detection. Hearing disorders are mainly due to a malfunction of the cochlea, the bone containing the cells inside the inner ear. Un ...

EPFL2019

Custom Adapter For Extended Field-Of-View Microsphere-Based Scanning Super-Resolution Microscopy

Martinus Gijs, Gergely Huszka

We present a dielectric microsphere-based super resolution optical microscope that can image a sample beyond the classical diffraction limit [1]. The super resolution imaging capability of a single microsphere normally is restricted to an area of similar t ...

IEEE2018

Dielectric-sphere-based microsystem for optical super-resolution imaging

Gergely Huszka

Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...

EPFL2018

Turning a normal microscope into a super-resolution instrument using a scanning microlens array

Martinus Gijs, Gergely Huszka

We report dielectric microsphere array-based optical super-resolution microscopy. A dielectric microsphere that is placed on a sample is known to generate a virtual image with resolution better than the optical diffraction limit. However, a limitation of s ...

2018

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

Single particle maximum likelihood reconstruction from superresolution microscopy images

Suliana Manley, Julia Gunzenhäuser

Point localization superresolution microscopy enables fluorescently tagged molecules to be imaged beyond the optical diffraction limit, reaching single molecule localization precisions down to a few nanometers. For small objects whose sizes are few times t ...

2017

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A dual-activatable, fluorogenic probe was developed to sense esterase activity with single-mol. resoln. Without enzymic preactivation, the diazoindanone-based probe has an electron-poor core and, upon irradn., undergoes Wolff rearrangement to give a ring-e ...

2017