Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli
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the signals involved in axonal trafficking and presynaptic clustering are poorly defined. Here we show that targeting of the -aminobutyric acid–synthesizing enzyme glutamate decarboxylase 65 (GAD65) to presynaptic clusters is mediated by its palmitoylated ...
The membrane-bound tetrachloroethene reductive dehalogenase (PCE-RDase) (PceA; EC 1.97.1.8), the terminal component of the respiratory chain of Dehalobacter restrictus, was purified 25-fold to apparent electrophoretic homogeneity. Sodium dodecyl sulfate-po ...
The detn. of residual dipolar couplings (RDCs) by quant. J spectroscopy methods such as heteronuclear single quantum correlation with phase encoded coupling (HSQC-PEC) is prone to systematic errors that may be caused by differential attenuation during the ...
Ligand field effects in lanthanide ions compounds have consequences for optical and magnetic spectroscopy. In the analysis of electron paramagnetic resonance spectra of Gd3+ complexes, a major role is played by the zero field splitting (ZFS), which is a hi ...
The translational diffusion coeff. of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the mols. is temporarily stored in the f ...
In the present paper, we present a complete characterization of the propylene sulfide-ethylene glycol triblock copolymers (PPS-PEG) at a mol. level, and as lyotropic aggregates, with the precise localization of the hydrophilic/hydrophobic border of their v ...
We present the design, fabrication and test of high-Q factor radiofrequency planar microcoils for nuclear magnetic resonance (NMR) spectroscopy in small volume samples. The coils are fabricated on glass wafers using high-aspect ratio SU-8 photoepoxy and co ...
The outer membrane protein OmpF from Escherichia coli is a member of a large family of b-barrel membrane proteins. Some, like OmpF, are pore-forming proteins while others are active transporters or enzymes. We have previously shown that the receptor-bindin ...
Measuring the nuclear magnetic resonance spectra of low-gamma heteronuclei such as N-15 constitutes an important analytical tool for the characterization of molecular structure and dynamics. The reduced resonance frequencies and magnetic moments of these h ...
The experimental performance of the phase modulated decoupling schemes SDROOPY-1 and SPINAL64 is investigated for heteronuclear dipolar decoupling in solid-state magic-angle-spinning nuclear magnetic resonance (NMR), in comparison with existing decoupling ...