Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli
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The atomic force microscope acquires topographs of single native membrane proteins at subnanometer resolution. Owing to the high signal-to-noise ratio, such images allow the conformational space of membrane protein surfaces to be sampled. This is demonstra ...
The detn. of residual dipolar couplings (RDCs) by quant. J spectroscopy methods such as heteronuclear single quantum correlation with phase encoded coupling (HSQC-PEC) is prone to systematic errors that may be caused by differential attenuation during the ...
The theory of single-transition cross-polarization in NMR is presented and verified by exptl. evidence. In comparison to conventional cross-polarization a qual. change in the mechanism is obsd. Under the influence of matched radiofrequency fields with ampl ...
2D P-31 refocused INADEQUATE NMR experiments have been used to determine through-bond P-O-P connectivities in crystalline and disordered phosphates. ...
During the membrane insertion process the major coat protein of bacteriophage M13 assumes a conformation in which two transmembrane helixes corresponding to the leader sequence and the anchor region in the mature part of the protein coming into close conta ...
In this article a method is suggested for restricting a sample (spatial localization) by preparing the magnetization with a phase-modulated radiofrequency pulse which inverts magnetization only over a very narrow range of radiofrequency field strengths. Th ...
Proteins from cisplatin treated bacterial cells were partially separated by 1D polyacrylamide gel electrophoresis and analysed by laser ablation inductively coupled plasma mass spectrometry; using peptide fingerprinting methods central to proteomics, the b ...
In this paper we describe the use of heteronuclear scalar couplings in solid-state NMR in order to generate multiple-quantum filtering (MQF) pulse sequences. These sequences can be used to edit CP/MAS spectra according to carbon multiplicity. Analytic expr ...
Gpi8p and Gaa1p are essential components of the GPI transamidase that adds glycosylphosphatidylinositols (GPIs) to newly synthesized proteins. After solubilization in 1.5% digitonin and separation by blue native PAGE, Gpi8p is found in 430-650-kDa protein ...
Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic path ...