Publication
Indication of 310-Helix Structure in Gas-Phase Neutral Pentaalanine
Publications associées (32)
Ion Spectroscopy Reveals Structural Difference for Proteins Microhydrated by Retention and Condensation of Water
Protein ubiquitin in its +7 charge state microhydrated by 5 and 10 water molecules has been interrogated in the gas phase by cold ion UV/IR spectroscopy. The complexes were formed either by condensing water onto the unfolded bare proteins in a temperature- ...
Washington2024Bridging Native and Intrinsic Structures of Microhydrated Biomolecules by Cold Ion Spectroscopy
Solving native structures of such large molecules, like biomolecules, is often challenging, particularly due to the potentially infinite number of non-covalent interactions with water. In this thesis, we report the use of cold ion gas-phase action spectros ...
EPFL2023Assessment of transferable forcefields for protein simulations attests improved description of disordered states and secondary structure propensities, and hints at multi-protein systems as the next challenge for optimization
Matteo Dal Peraro, Luciano Andres Abriata
Continuous assessment of transferable forcefields for molecular simulations is essential to identify their weaknesses and direct improvement efforts. The latest efforts focused on better describing disordered proteins while retaining proper description of ...
2021Mid-Infrared plasmonic nanoantennas for ultrasensitive detection of proteins and their secondary structure
Engineered plasmonic nanostructures have emerged as powerful sensing platforms and are promoting novel applications in various fields. By providing extreme field confinement down to molecular dimensions and enhancing light-matter interaction at the nanosca ...
EPFL2018Quantifying the Limits of Detection of Surface-Enhanced Infrared Spectroscopy with Grating Order-Coupled Nanogap Antennas
Hatice Altug, Andreas Tittl, Aurélian Michel John-Herpin
Infrared spectroscopy is widely used for biomolecular studies, but struggles when investigating minute quantities of analytes due to the mismatch between vibrational cross sections and IR wavelengths. It is therefore beneficial to enhance absorption signal ...
2018Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection
Giovanni Dietler, Hilal Lashuel, Hatice Altug, Francesco Simone Ruggeri, Dordaneh Etezadi, John Blaine Warner IV
Plasmonic nanoantennas offer new applications in mid-infrared (mid-IR) absorption spectroscopy with ultrasensitive detection of structural signatures of biomolecules, such as proteins, due to their strong resonant near-fields. The amide I fingerprint of a ...
Springer Nature2017Gas-phase probes of kinetically trapped peptides
Gas-phase techniques are promising for studying intrinsically disordered peptides and proteins, as they can be implemented in a conformer-selective way. One of the issues that prevent them from finding a broader use is the question of to which extent the s ...
EPFL2016AFM-Based Single Molecule Techniques: Unraveling the Amyloid Pathogenic Species
Giovanni Dietler, Francesco Simone Ruggeri, Andrea Cerreta
Background: A wide class of human diseases and neurodegenerative disorders, such as Alzheimer's disease, is due to the failure of a specific peptide or protein to keep its native functional conformational state and to undergo a conformational change into a ...
Bentham Science Publishers2016Testing the diffusing boundary model for the helix-coil transition in peptides
The dynamics of peptide α-helices have been studied extensively for many years, and the kinetic mechanism of the helix-coil dynamics has been discussed controversially. Recent experimental results have suggested that equilibrium helix-coil dynamics are gov ...
2013Structural Studies and Protein Engineering of Human O6-Alkylguanine-DNA Alkyltransferase
The specific labeling of proteins with synthetic probes is a powerful approach to study protein function and protein tags have been widely used for this purpose. A well-established example for a self-labeling protein tag is SNAP-tag. It specifically reacts ...
EPFL2012