Publication
Method for hormone or drug screening in a tissue sample
Publications associées (37)
A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
Christoph Bostedt, Camila Bacellar Cases Da Silveira
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such experiments. Here, we ...
Berlin2023DUBS: A Framework for Developing Directory of Useful Benchmarking Sets for Virtual Screening
Benchmarking is a crucial step in evaluating virtual screening methods for drug discovery. One major issue that arises among benchmarking data sets is a lack of a standardized format for representing the protein and ligand structures used to benchmark the ...
2020Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms
Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Elena Katsyuba, Hüseyin Baris Atakan, Rongrong Xiang
The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...
2019A Universal Assay for Aminopeptidase Activity and Its Application for Dipeptidyl Peptidase-4 Drug Discovery
Gerardo Turcatti, Marc Chambon, Julien Bortoli Chapalay, Elena Goun, Jonathan Patrice Vesin, Arkadiy Bazhin, James William Collins
Aminopeptidases, such as dipeptidyl peptidase 4 (DPP-4, CD26), are potent therapeutic targets for pharmacological interventions because they play key roles in many important pathological pathways. To analyze amino-peptidase activity in vitro (including hig ...
AMER CHEMICAL SOC2019A novel cell-based sensor detecting the activity of individual basic proprotein convertases
The basic proprotein convertases (PCs) furin, PC1/3, PC2, PC5/6, PACE4, PC4, and PC7 are promising drug targets for human diseases. However, developing selective inhibitors remains challenging due to overlapping substrate recognition motifs and limited str ...
WILEY2019An optimized and validated 384-well plate assay to test platelet function in a high-throughput screening format
Nikolaos Stergiopoulos, Olivier Burri, Marc Chambon, Julien Bortoli Chapalay, Fabiana Pereira Da Costa Fraga, Augusto Martins Lima, Maiia Bragina
Despite significant advances in the treatment of cardiovascular diseases, antiplatelet therapies are still associated with a high risk of hemorrhage. In order to develop new drugs, methods to measure platelet function must be adapted for the high-throughpu ...
TAYLOR & FRANCIS INC2019Multimodal imaging and high-throughput image-processing for drug screening on living organisms on-chip
Michaël Unser, Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Daniel Migliozzi, Virginie Sophie Uhlmann
A major step for the validation of medical drugs is the screening on whole organisms, which gives the systemic information that is missing when using cellular models. Caenorhabditis elegans is a soil worm that catches the interest of researchers who study ...
2019A microfluidics platform for combinatorial drug screening on cancer biopsies
Screening drugs on patient biopsies from solid tumours has immense potential, but is challenging due to the small amount of available material. To address this, we present here a plug-based microfluidics platform for functional screening of drug combinatio ...
2018Novel compartment-specific biosensors reveal a complementary subcellular distribution of bioactive Furin and PC7
Proprotein Convertases (PCs) constitute a family of serine proteases that activate various
hormones, growth factors and cell adhesion molecules by mediating endoproteolytic cleavage
of their secreted inactive precursors during their transit in the secret ...
EPFL2018Pancreatic α- and β-Cellular Clocks Have Distinct Molecular Properties and Impact on Islet Hormone Secretion and Gene Expression
Michaël Unser, Daniel Sage, Cédric Gobet, Frédéric Bruno Martin Gachon
A critical role of circadian oscillators in orchestrating insulin secretion and islet gene transcription has been demonstrated recently. However, these studies focused on whole islets and did not explore the interplay between α-cell and β-cell clocks. We p ...
2017