Publication
Structural heterogeneity of the ion and lipid channel TMEM16F
Publications associées (36)
Prospects of Observing Ionic Coulomb Blockade in Artificial Ion Confinements
Aleksandra Radenovic, Andrey Chernev, Sanjin Marion
Nanofluidics encompasses a wide range of advanced approaches to study charge and mass transport at the nanoscale. Modern technologies allow us to develop and improve artificial nanofluidic platforms that confine ions in a way similar to single-ion channels ...
MDPI2020An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel
Cyclic nucleotide-gated (CNG) ion channels are non-selective cation channels key to signal transduction. The free energy difference of cyclic-nucleotide (cAMP/cGMP) binding/unbinding is translated into mechanical work to modulate the open/closed probabilit ...
Springer Science and Business Media LLC2018A Gating Mechanism of the Serotonin 5-HT3 Receptor
Our recently solved high-resolution structure of the serotonin 5-HT3 receptor (5-HT3R) delivered the first detailed structural insights for a mammalian pentameric ligand-gated ion channel. Based on this structure, we here performed a total of 2.8-μs all-at ...
Cell Press2016Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels
Eukaryotic cyclic nucleotide-modulated (CNM) ion channels perform various physiological roles by opening in response to cyclic nucleotides binding to a specialized cyclic nucleotide-binding domain. Despite progress in structure-function analysis, the confo ...
Springer Nature2016On the role of water density fluctuations in the inhibition of a proton channel
Lucie Monique Françoise Delemotte
Hv1 is a transmembrane four-helix bundle that transports protons in a voltage-controlled manner. Its crucial role in many pathological conditions, including cancer and ischemic brain damage, makes Hv1 a promising drug target. Starting from the recently sol ...
Natl Acad Sciences2016The Structure of the Mouse Serotonin 5-HT3 Receptor in Lipid Vesicles
Horst Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Luigino Grasso, Ghérici Hassaïne
The function of membrane proteins is best understood if their structure in the lipid membrane is known. Here, we determined the structure of the mouse serotonin 5-HT3 receptor inserted in lipid bilayers to a resolution of 12 Å without stabilizing antibodie ...
Elsevier2016A KcsA/MloK1 Chimeric Ion Channel Has Lipid-dependent Ligand-binding Energetics
Background: The mechanism of ligand gating in physiologically important cyclic nucleotide-modulated channels is unknown. Results: We constructed and purified a chimeric ion channel with activity modulated by cAMP and used it to measure ligand-binding energ ...
American Society for Biochemistry & Molecular Biology (ASBMB)2014Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1
Cyclic nucleotide-modulated ion channels are important for signal transduction and pacemaking in eukaryotes. The molecular determinants of ligand gating in these channels are still unknown, mainly because of a lack of direct structural information. Here we ...
Springer Nature2014Thermal Unfolding of a Mammalian Pentameric Ligand-gated Ion Channel Proceeds at Consecutive, Distinct Steps
Horst Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Ghérici Hassaïne, Menno Berend Tol
Pentameric ligand-gated ion channels (LGICs) play an important role in fast synaptic signal transduction. Binding of agonists to the beta-sheet-structured extracellular domain opens an ion channel in the transmembrane alpha-helical region of the LGIC. How ...
Amer Soc Biochemistry Molecular Biology Inc2013A Biochemical and Biophysical Investigation of 5-HT3 Receptor Stability
The 5-hydroxytryptamine 3 receptor (5-HT3R) is a member of the pentameric ligand-gated ion channel (pLGIC) family, that plays an important role in fast signal transduction and cell-cell communication in synapses: They convert a chemical signal from a neuro ...
EPFL2012