Publication
A Multiresolution Approach to Orientation Assignment in 3D Electron Microscopy of Single Particles
Publications associées (73)
Microsecond Time-Resolved Cryo-Electron Microscopy
Recently, single-particle cryo-electron microscopy emerged as a technique capable of determining protein structures at near-atomic resolution and resolving protein dynamics with a temporal resolution ranging from second to milliseconds. This thesis describ ...
EPFL2024Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets
Volume electron microscopy is the method of choice for the in situ interrogation of cellular ultrastructure at the nanometer scale, and with the increase in large raw image datasets generated, improving computational strategies for image segmentation and s ...
Berlin20243D reconstruction of curvilinear one-dimensional objects viewed in transmission electron microscopy
To obtain a more complete understanding of material microstructure at the nanoscale and to gain profound insights into their properties, there is a growing need for more efficient and precise methods that can streamline the process of 3D imaging using a tr ...
EPFL2023Towards near-atomic resolution imaging of microsecond nanoscale dynamics
Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...
EPFL2023In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes
Observing the fast dynamics of nanoscale systems is crucial in order to understand and ultimately control their behavior. Characterizing these dynamic processes requires techniques with atomic spatial resolution and a temporal resolution that matches the t ...
EPFL2023Correlating cryo-super resolution radial fluctuations and dual-axis cryo-scanning transmission electron tomography to bridge the light-electron resolution gap
Visualization of organelles and their interactions with other features in the native cell remains a challenge in modern biology. We have introduced cryo-scanning transmission electron tomography (CSTET), which can ac-cess 3D volumes on the scale of 1 micro ...
ACADEMIC PRESS INC ELSEVIER SCIENCE2023One-Dimensional "Ghost Imaging" in Electron Microscopy of Inelastically Scattered Electrons
Fabrizio Carbone, Giovanni Maria Vanacore, Ivan Madan, Ido Kaminer, Simone Gargiulo, Ebrahim Karimi
Entanglement and correlation are at the basis of quantummechanicsand have been used in optics to create a framework for "ghostimaging". We propose that a similar scheme can be used in anelectron microscope to exploit the correlation of electrons with theco ...
AMER CHEMICAL SOC2023Machine-Learning-Enhanced Procedural Modeling for 4D Historical Cities Reconstruction
Frédéric Kaplan, Isabella Di Lenardo, Rémi Guillaume Petitpierre, Beatrice Vaienti
The generation of 3D models depicting cities in the past holds great potential for documentation and educational purposes. However, it is often hindered by incomplete historical data and the specialized expertise required. To address these challenges, we p ...
2023Random conical tilt reconstruction without particle picking in cryo-electron microscopy
A method is proposed to reconstruct the 3D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. The method uses autocorrelation analysis on the micrographs to estimate f ...
INT UNION CRYSTALLOGRAPHY2022Bragg edge tomography characterization of additively manufactured 316L steel
Roland Logé, Christian Leinenbach, Efthymios Polatidis, Markus Strobl, Nikola Kalentics
In this work we perform a neutron Bragg edge tomography of stainless steel 316L additive manufacturing samples, one as built via standard laser powder bed fusion, and one using the novel three-dimensional (3D) laser shock peening technique. First, we consi ...
AMER PHYSICAL SOC2022