Process development for the production of monoclonal IgA antibodies that protect mucosal surfaces
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Monoclonal IgA antibodies have been produced by a hybridoma cell line grown in different bioreactors using serum-contg. and protein-free media in both basal and fortified versions. The effects of culture conditions on IgA prodn. and quality were studied us ...
A chemically-defined protein-free medium (FMX-Turbodoma) has been improved for the production of monoclonal IgA antibodies by hybridoma cells, using a systematic method. Cell growth rate, IgA production, activity and molecular weight pattern have been used ...
The asym. synthesis of two appropriately functionalized nonproteinogenic amino acids I (Troc = CO2CH2CCl3, TBS = SiMe2CMe3) and II (Boc = Me3CO2C) needed for the total synthesis of vancomycin is described. The assemblage of these amino acids into linear tr ...
Immobilized lactose of A. oryzae was used for the hydrolysis of lactose in partially deproteinized whey in a fluidized-bed reactor packed with motionless mixers. The lactase was immobilized on chitosan-coated silica gel particles by means of glutaraldehyde ...
199Hg NMR spectroscopy is used to examine the interaction of the toxic organomercurial ethylmercury phosphate [2235-25-8] with various amino acids. Because of the relative insensitivity of 199Hg and the low concns. of biol. mols., a heteronuclear spin-echo ...
A review with many refs. Ammonia has been reported to be toxic and inhibitory for mammalian cell cultures for many years. Redn. of growth rates and maximal cell densities in batch cultures, changes in metabolic rates, perturbation of protein processing and ...
A kidney cortex cDNA clone (rBAT) has recently been isolated, which upon in vitro transcription and capping complementary RNA (cRNA) and injection into Xenopus laevis oocytes induces a system b0,(+)-like amino acid transport activity. This cDNA encodes a t ...
Vasoactive intestinal polypeptide (VIP) was labeled with sodium [125I]iodide using the chloramine-T method and subsequently purified by reverse-phase high performance liquid chromatography. Three main 125I-labeled peaks designated A, B, and C resulted from ...