Towards the generation of artificial O6-alkylguanine-DNA alkyltransferases: In vitro selection of antibodies with reactive cysteine residues
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The molecular processes based on the specific receptor-ligand interactions (e.g. immune response to a foreign antigen, communication between nerve cells, etc.) are essential for a good and stable performance of living organisms. The aim of the present work ...
Monoclonal antibodies (Mabs) are valuable reagents for the purification, characterization and immunolocalization of proteins.In this study, we raised Mabs against human peroxisome proliferator-activated receptors (PPARs) using baculovirus particles display ...
The DNA binding domain (DBD) is the most mutated region of p53 in tumors and has proven to be relatively resistant to the generation of specific antibodies. Template assembled synthetic peptide (TASP) synthesis of a peptide derived from the DBD creates a h ...
Chem. ligation methods in combination with regioselective addressable templates have allowed the prepn. of well defined complex monomol. films tethered to gold surfaces. The supramol. monolayers exposed artificial recognition sites, here for monoclonal ant ...
Highly efficient capillary electrochromatog. sepns. of cardiac glycosides and other steroids are presented. Employing butyl-derivatized silica particles as stationary phase resulted in a nearly three times faster electroosmotic flow (EOF) compared to capil ...
The first part of this work deals with oxidative addition reactions of the triangulo platinum cluster [Pt3(μ-CO)3(PCy3)3]. Reactions with the electrophilic molecules ICH2CN and X2 (X = I and Br) and with the weakly polar reagent Ph3SnH have been studied. A ...
A unified strategy for the synthesis of mauritines A (I), B, C, and F has been developed based on a key intramol. nucleophilic arom. substitution reaction (SNAr) for the formation of the strained 14-membered paracyclophane. It was demonstrated that the out ...
The encounter between anisotropic agents in diffusion-controlled reactions is a topic of very general relevance in chemistry and biology. Here we introduce a simplified model of encounter of an isotropic molecule with a pair of partially reacting agents an ...
The smaller isoform of the GABA-synthesizing enzyme, glutamic acid decarboxylase 65 (GAD65), is unusually susceptible to becoming a target of autoimmunity affecting its major sites of expression, GABA-ergic neurons and pancreatic β-cells. In contrast, a hi ...
The on-line electrochemical tagging (EC-tagging) of cysteine residues in proteins during mass spectrometry is studied to probe the cysteine environment. Benzoquinone probes electrogenerated at a microspray electrode react with the thiol functions of the pr ...