Protein Mass Spectrometry and Proteomics

This lecture covers the principles of liquid chromatography, focusing on factors affecting resolution, capacity, and asymmetric peaks. It also delves into various chromatographic techniques such as adsorption, reversed-phase, ion exchange, and affinity chromatography. The instructor explains the use of high-performance liquid chromatography (HPLC) and its components, detectors, and different setups. Additionally, the lecture discusses immobilized metal affinity chromatography (IMAC) and the advantages of capillary and nano-LC. The comparison between off-gel isoelectric focusing (IEF) and strong cation exchange (SCX) chromatography is explored, highlighting their complementarities for in-depth proteome coverage.