Confocal Microscopy: Imaging Techniques and Applications

This lecture covers the advantages of confocal imaging, including optical sectioning, z-series construction, 3D visualization, and image quality factors such as spatial and temporal resolution. It also discusses the principles of confocal microscopy, the role of the pinhole, and trade-offs in image quality. The instructor demonstrates the use of confocal microscopy for volumetric imaging, cross-section analysis, and 3D visualization of biological samples, emphasizing the benefits over conventional light microscopy. Various techniques for improving resolution and image quality are explored, providing insights into the practical considerations and limitations of confocal microscopy.