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This lecture on Mass Spectrometry covers the principles of ionization, mass sorting, and analysis according to mass/charge ratio. It explains the operation of various ion sources, analyzers, and detectors, such as Electron Ionization, MALDI, and Quadrupole Mass Filters. The instructor discusses the advantages and disadvantages of different ionization techniques, including Electron Spray Ionization and Chemical Ionization. The lecture also delves into the functioning of instruments like Linear Time-of-Flight and Reflectron Time-of-Flight mass analyzers, highlighting their capabilities and limitations.