Programmable RNA-Targeting & Genome Editing

This lecture by the instructor covers the principles of synthetic biology, focusing on programmable RNA-targeting and genome editing using CRISPR technology. It delves into the history of CRISPR, its adaptive immune system in prokaryotes, and the mechanisms of CRISPR-Cas9 cleavage. The lecture also explores precision RNA technologies, base editing strategies, and the applications of CRISPR-based diagnostics, particularly in the context of SARS-CoV-2. Additionally, it discusses the methodology for programmable RNA targeting, protein-based RNA effectors, and the SHERLOCK detection assay for CoVID-19. The lecture concludes with insights into the significance of CRISPR-Cas9 in genome engineering and its diverse applications in various fields.