CRISPR-Based Tools & Diagnostics

This lecture explores CRISPR-based tools and diagnostics, focusing on the development of faster, simpler, and cheaper testing methods with high specificity and sensitivity. The history of CRISPR, its adaptive immune system in prokaryotes, and the mechanisms of Cas9 and Cas13 are discussed. The lecture delves into strategies for base editing in eukaryotic genomes, the popularity of CRISPR-Cas system in genome engineering, and the potential of CRISPR-based diagnostics for diseases like SARS-CoV-2. Advanced concepts include the SHERLOCK detection assay and the integration of internal controls for RNAse contamination.