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This lecture explores metabolic pathways such as the hydroxypropionate bi-cycle and the 3-hydroxypropionate/4-hydroxybutyrate cycle, used by various organisms for CO2 fixation. It delves into the energy-rich compounds involved in substrate-level phosphorylation and the different fermentation processes, highlighting the importance of biofuels as an alternative to fossil fuels. The instructor discusses the Embden Meyerhof Parnas pathway, the Calvin-Benson-Bassham cycle, and the Reductive citric acid cycle, emphasizing the role of key enzymes and organisms in these pathways.