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Concept
Insertion (genetics)
Summary
In genetics, an insertion (also called an insertion mutation) is the addition of one or more nucleotide base pairs into a DNA sequence. This can often happen in microsatellite regions due to the DNA polymerase slipping. Insertions can be anywhere in size from one base pair incorrectly inserted into a DNA sequence to a section of one chromosome inserted into another. The mechanism of the smallest single base insertion mutations is believed to be through base-pair separation between the template and primer strands followed by non-neighbor base stacking, which can occur locally within the DNA polymerase active site. On a chromosome level, an insertion refers to the insertion of a larger sequence into a chromosome. This can happen due to unequal crossover during meiosis.
N region addition is the addition of non-coded nucleotides during recombination by terminal deoxynucleotidyl transferase.
P nucleotide insertion is the insertion of palindromic sequences encoded by the ends of the recombining gene segments.
Trinucleotide repeats are classified as insertion mutations and sometimes as a separate class of mutations.
Zinc finger nuclease(ZFN), Transcription activator-like effector nucleases (TALEN), and CRISPR gene editing are the three main methods used in the former research to achieve gene insertion. And CRISPR/Cas tools have already become one of the most used methods to present research.
Based on CRISPR/Cas tools, different systems have already been developed to achieve specific functions. For example, one strategy is double-strand nucleases cutting system, using the normal Cas9 protein with single guide RNA (sgRNA) and then achieving the gene insertion through end-joining or dividing cells with the DNA repair system. Another example is the prime editing system, which uses Cas9 nickase and the prime editing guide RNA (pegRNA) carrying the target genes.
One limitation of current technology is that the size for DNA precise insertion is not large enough to meet the demand for genome research.
Official source
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