Coulter counter

A Coulter counter is an apparatus for counting and sizing particles suspended in electrolytes. The Coulter counter is the commercial term for the technique known as resistive pulse sensing or electrical zone sensing. The apparatus is based on the Coulter principle named after its inventor, Wallace H. Coulter. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions. As fluid that contains particles or cells is drawn through the microchannels, each particle causes a brief change to the electrical resistance of the liquid. The counter detects these changes in the electrical resistance. Coulter principle The Coulter principle states that particles pulled through an orifice, concurrent with an electric current, produce a change in impedance proportional to the volume of the particle traversing the orifice. This pulse in impedance originates from the displacement of electrolyte caused by the particle. The principle ha

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MICRO-560: BioMEMS

This course covers the main applications of micro devices for life science and biomedical applications. The course is organized by application topic. It is also covering the basic physical, biological, chemical, technological concepts, which are presented as transversal introductory section

Node-Pore Coded Coincidence Correction: Coulter Counters, Code Design, and Sparse Deconvolution

François Réal Rivest

2018

Impedance-Based Single-Cell Pipetting

Yann Barrandon, Nicolas Jean Bernard Beuchat, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Philippe Renaud, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.

SAGE PUBLICATIONS INC2020

Tip connector for fluidic and electrical connection

Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, David Forchelet, Philippe Renaud, Nicolas Uffer

A connector for providing both a fluidic and electrical connection is disclosed, said connector having a proximal end, a distal end and an elongated body in between, characterized in that the elongated body has an inner cavity spanning throughout its length, and the distal end comprises: a) an inner body portion comprising a first inner electrical contact and b) an outer body portion comprising a second outer electrical contact having a spring element. The connector is easily adaptable to many kind of fluidic actuators and particularly to pipette instruments usually found in laboratory practice and developed to adapt fluidic actuators and pipette instalments to work according to the Coulter principle in every working condition, in particular to adapt the electrical and fluidic connection between a sensing tip and an instrumented pipette.

2018

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Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is s

A lab-on-a-chip (LOC) is a device that integrates one or several laboratory functions on a single integrated circuit (commonly called a "chip") of only millimeters to a few square centimeters to ach