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Mass Spectrometry: Techniques and Applications
This lecture covers the principles and applications of mass spectrometry, focusing on techniques such as Electron Ionization (El-MS), Chemical Ionization (CI-MS), Fast Atom Bombardment (FAB-MS), Electrospray (ESI-MS), and Matrix Assisted Laser Desorption Ionization (MALDI). It explains how mass spectrometry can provide information on molecular composition and structure through the analysis of isotopic distribution, fragmentation patterns, and ionization methods. The lecture also discusses the importance of isotopic profiles in identifying molecules and proteins, showcasing real examples of mass spectra and the role of mass spectrometry in proteomics.