Protein Mass Spectrometry and Proteomics

This lecture covers the fundamentals of protein mass spectrometry and proteomics, including an introduction to protein analysis, mass spectrometry reminders, ionization sources, analyzers, and detectors used in proteomics. It also discusses proteomic strategies, workflows, and separation techniques, emphasizing the importance of mass accuracy and resolution in identifying compounds. The instructor delves into the concepts of mass defect, isotopes, charges, and the need for high resolving power. Various ionization methods such as electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are explained, along with the significance of mass analyzers in proteomics.