DNA Replication: Enzymes and Mechanisms

This lecture delves into the intricate process of DNA replication, focusing on the role of enzymes such as DNA polymerase and helicase. The instructor explains how DNA polymerase creates phosphodiester bonds and ensures accuracy by selecting the correct nucleotides. The lecture also covers the function of DNA gyrase in preventing supercoiling and the motor protein nature of helicase. Through detailed animations, the audience gains insight into the energy-dependent movement of helicase on DNA strands. The presentation concludes with a discussion on the Polymerase Chain Reaction (PCR) as a tool for replicating DNA in a laboratory setting.