Super Resolution Microscopy: STED Method

This lecture covers the principles and applications of Super Resolution Microscopy, focusing on the Stimulated Emission Depletion (STED) method. It explains the key parameters and optimization techniques for achieving high-resolution imaging, including the role of STED laser intensity and saturation intensity of the fluorophore. The instructor discusses the non-linear dependence of the depleted population of excited electrons on the STED laser beam intensity, leading to super-resolution imaging. Examples of suitable dyes and lasers for STED imaging are provided, along with the resolution factors and limitations of STED microscopy.