Fluorescence: High Contrast Imaging Technique

This lecture covers the principles of fluorescence microscopy, including the use of fluorescent dyes to tag non-fluorescent entities for visibility. It explains how fluorescence provides high contrast, specificity, and allows for quantitative monitoring. The lecture also discusses the implementation of fluorescence microscopy, the specificity achieved at the molecular level, and examples of bio-applications. Additionally, it delves into the quantum nature of light, the photoelectric effect, and the observation of photon absorption and emission in a 2-level system.