Enzymes' based Biosensors

This lecture covers the principles of enzymes' based biosensors, focusing on the immobilization of enzymes on conductive substrates to generate electrical signals through biochemical reactions. Topics include redox reactions with oxidases and cytochromes, hexokinases based biosensors for ATP detection, and amperometric DNA detection. The lecture also discusses the working principles of P450 enzymes, enzymatic cleavage, redox processes, and the detection of hydrogen peroxide. It explains the concept of sensitivity in biosensors, the difference between geometrical and active areas in electrochemical cells, and the limit of detection. The Clarke's Error Grid and non-linear biosensors are also addressed.