Lecture
Protein Purification: IMAC
In course
DEMO: tempor velit
Pariatur commodo ut nisi aute proident magna esse dolore ad Lorem cillum sunt. Enim ad quis exercitation quis esse aute duis et aliqua do sunt. Elit fugiat irure voluptate pariatur culpa magna minim est do qui qui consectetur. Voluptate exercitation pariatur eu eiusmod in quis laboris et dolore quis ad et. Commodo elit sunt cupidatat sit reprehenderit sint ullamco magna velit excepteur deserunt occaecat consectetur nulla.
Description
This lecture covers the purification of proteins using immobilized metal-ion chromatography (IMAC). Starting with the transformation of E. coli and lysis of bacteria, the instructor explains the process step by step, including binding, washing, and elution in IMAC purification. The analysis of the purified protein and the use of a fluorescent protein database for identification are also discussed.
Instructor
officia irure laborum tempor
Eiusmod amet consequat exercitation aliqua eu ipsum reprehenderit ullamco adipisicing voluptate qui irure laborum. Laboris enim consectetur id elit ut irure irure aute. Est minim consequat consequat Lorem nulla esse adipisicing excepteur laborum irure nisi dolor.
Official source
About this result
This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.
Related lectures (33)
Chemical Biology Tools: DDX3 Mutant Analysis
Analyzes a mutant DDX3 enzyme's ATPase activity and inhibitor development, covering RNA chaperones, crystal structures, and mass spectrometry-based protein profiling.
Protein Expression & Purification
Explores protein expression, purification, and gel filtration for isolating protein complexes.
Protein Purification Methods
Covers the purification of expressed proteins using methods like transformation, lysis, IMAC, and protein analysis.
Protein Mass Spectrometry and Proteomics: Techniques and Applications
Explores protein mass spectrometry techniques, including labeling methods, quantitation, and biomarker discovery.
Self-attraction of DNA: Mediated Interactions
Discusses the self-attraction of DNA and its mediated interactions.