Protein Purification: IMAC

In course

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Description

This lecture covers the purification of proteins using immobilized metal-ion chromatography (IMAC). Starting with the transformation of E. coli and lysis of bacteria, the instructor explains the process step by step, including binding, washing, and elution in IMAC purification. The analysis of the purified protein and the use of a fluorescent protein database for identification are also discussed.

Instructor

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Official source

https://mediaspace.epfl.ch/media/0_igc6mxq9

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