Lecture
This lecture covers the principles and methods of protein chromatography for the purification of proteins. It explains the importance of knowing the protein's location in a fraction, the types of chromatographic methods used, such as column chromatography, and the steps involved in the process. The lecture also delves into affinity chromatography, discussing common tags used, fusion protein construction strategies, and the process of affinity purification. Additionally, it explores the cleavage sites for removing tags using proteases and the analysis of collected fractions. The lecture concludes with key take-home messages on recombinant protein expression, different expression systems, and the main chromatographic techniques in protein purification.