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This lecture covers the principles and applications of tomography, a method used in various fields such as medicine, biology, and materials science, for reconstructing 3D structures from 2D projections. It explains the process of tomographic reconstruction, the historical background, and showcases examples of direct tomography using techniques like FIB Nanotomography. The lecture also delves into advanced topics like HAADF-STEM imaging for materials science, discussing concepts such as z-contrast and high angle incoherent scattering. The instructor emphasizes the importance of non-destructive tomography methods and the role of tomography in advancing scientific research.