Quantitative Proteome Profiling: Chemical Target Identification

This lecture delves into the quantitative profiling of the global proteome to identify potential drug targets through activity-based protein profiling (ABPP). It covers methods like MS-based protein profiling, target enrichment, and competitive ABPP. The instructor explains the workflow of bottom-up mass spectrometry and the use of stable isotope labeling for quantitative target identification. Advanced concepts such as Tandem Mass Tag (TMT) labeling and isoTOP-ABPP are also discussed. The lecture emphasizes the importance of understanding protein reactivity and functional signaling output for drug discovery.