Protein Profiling: Mass Spectrometry and Target-ID Tools

This lecture covers the use of mass spectrometry for protein profiling, focusing on the bottom-up and top-down approaches. It explains the process of protein digestion, peptide analysis, and the identification of protein structures. Additionally, it discusses target enrichment techniques such as affinity pulldown and biotin-streptavidin pulldown, as well as the application of click coupling chemistry. The lecture also introduces stable isotope labeling for quantitative proteomics and the SILAC protocol for identifying small-molecule interactions with proteins.