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Lecture
Characterization of Proteins by Electrophoresis
Description
This lecture covers the physics behind the electrophoresis technique used for the characterization of proteins, including Native Gel, SDS-PAGE, and 2D Gel. It explains the different types of gels commonly used, the range of separation for DNA and proteins, and the stains used for biopolymers after electrophoretic separation. The lecture also delves into isotachophoresis within the stacking gel zone and the principles of SDS-PAGE in denaturing and non-denaturing conditions.
Official source
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