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By Dr. Luca Chiapperino delves into the implications of post-genomics on health and society, focusing on the molecularization of our biography, experiences, and living environment.
Covers the historical development of genomics, from Sanger sequencing to high-throughput technologies and cost reductions, highlighting the impact of key players like Craig Venter.
Explores the evolution of DNA sequencing techniques, from shotgun sequencing to real-time PCR, emphasizing the importance of direct RNA sequencing and the challenges of repetitive DNA sequences.
Introduces BioMEMS, covering applications like DNA separation, protein analysis, and neuroelectronic implants, along with key numbers in biology and challenges in system integration.
Explores single molecule sequencing strategies, including sequencing by synthesis and real-time sequencing based on zero mode waveguides, as well as DNA translocation through nanopores.
Explores the applications of CRISPR-Cas in genome editing, focusing on engineering bacterial genomes, curing genetic diseases, guide RNA simplicity, Cas9 specificity, DNA damage mechanisms, and base editing.
