Fluorescence Microscopy: Imaging Techniques

This lecture covers the various imaging techniques in fluorescence microscopy, including contrast adjustment, specificity achieved at the molecular level, and the implementation of fluorescence for monitoring. It also delves into the principles of FRET (Förster Resonance Energy Transfer), its main requirements, typical values, and configurations in biology applications. The lecture emphasizes the importance of fluorophore proximity, spectral overlap, dipole alignment, and favorable environmental conditions in FRET. Additionally, it explores the advantages and disadvantages of using FRET in biological studies.