Protein Mass Spectrometry and Proteomics: Search Engines and Database Algorithms

This lecture covers the principles of protein mass spectrometry and proteomics, focusing on search engines like Mascot and database algorithms. It explains the conversion of mass spectra into peak lists, identification of proteins, and quantification methods. Various search software such as Andromeda, Byonic, and Comet are discussed, along with their features and applications. The lecture also delves into validation techniques, false discovery rate estimation, and relative quantification using isobaric tagging data.