This lecture covers the concept of buffer solutions in biology, including the Henderson-Hasselbalch equation, properties of buffer mixtures, and examples like the acetate buffer system. It also discusses the physiological importance of buffer systems in maintaining the pH balance in blood and cells, as well as the role of respiratory and renal regulation in pH control. Additionally, it explores the denaturation of proteins, the use of physiological buffers like Tris-HCl, and the preparation of buffer solutions with specific pH values.