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This lecture covers the formation of droplets using microfluidic confinement, the role of surfactants in stabilizing droplets, and different methods for droplet manipulation such as capillary instability, step emulsification, and trapping of droplets. It also discusses the encapsulation of cells in droplets, single-cell encapsulation techniques, and high-throughput single-cell protein profiling. Various concepts like hydrophobic walls, surfactant properties, capillary forces, and Dean flow are explained in detail.