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Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

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We present a new method for lysis of single cells in continuous flow, where cells are sequentially trapped, lysed and released in an automatic process. Using optimized frequencies, dielectrophoretic trapping allows exposing cells in a reproducible way to h ...

Elsevier2012

Background: Fluorescence loss in photobleaching (FLIP) is a widely used imaging technique, which provides information about protein dynamics in various cellular regions. In FLIP, a small cellular region is repeatedly illuminated by an intense laser pulse, ...

Biomed Central Ltd2012

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

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Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maxim ...

Springer Netherlands2011

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Photoactivatable Probes for Protein Labeling

Sambashiva Banala

Over the last decade, several protein tags have been developed for the specific and covalent labeling of fusion proteins with small organic probes. Among them, SNAP-tag has been used in many applications such as live cell imaging, protein microarrays and t ...

EPFL2010

Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids

Marcel Leutenegger

We describe an optical method capable of tracking a single fluorescent molecule with a flexible choice of high spatial accuracy (~10–20 nm standard deviation or ~20–40 nm full-width-at-half-maximum) and temporal resolution (< 1 ms). The fluorescence signal ...

National Academy of Sciences2010

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The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

We demonstrate nanometer-level localization accuracy of a single fluorescent emitter in three dimensions. Our super resolution microscopy technique is based on spectral self-interference for axial localization and two-dimensional diffraction pattern analys ...

2009

A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...

2008