Cryogenic electron microscopyCryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.
HunanHunan (UKhuː'næn, UShuː'nɑːn; ) is a landlocked province of the People's Republic of China, part of the South Central China region. Located in the middle reaches of the Yangtze watershed, it borders the province-level divisions of Hubei to the north, Jiangxi to the east, Guangdong and Guangxi to the south, Guizhou to the west and Chongqing to the northwest. Its capital and largest city is Changsha, which also abuts the Xiang River. Hengyang, Zhuzhou, and Yueyang are among its most populous urban cities.
Linear equationIn mathematics, a linear equation is an equation that may be put in the form where are the variables (or unknowns), and are the coefficients, which are often real numbers. The coefficients may be considered as parameters of the equation, and may be arbitrary expressions, provided they do not contain any of the variables. To yield a meaningful equation, the coefficients are required to not all be zero. Alternatively, a linear equation can be obtained by equating to zero a linear polynomial over some field, from which the coefficients are taken.
Transmission electron cryomicroscopyTransmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
MaldivesThe Maldives (ˈmɑːldivz ; ދިވެހިރާއްޖެ, diʋehi ɾaːd͡ʒːe), officially the Republic of Maldives (ދިވެހިރާއްޖޭގެ ޖުމްހޫރިއްޔާ, diʋehi ɾaːd͡ʒːeːge d͡ʒumhuːɾijjaː), is an archipelagic state and country in South Asia, situated in the Indian Ocean. It lies southwest of Sri Lanka and India, about from the Asian continent's mainland. The Maldives' chain of 26 atolls stretches across the equator from Ihavandhippolhu Atoll in the north to Addu Atoll in the south.
Stimulated emissionStimulated emission is the process by which an incoming photon of a specific frequency can interact with an excited atomic electron (or other excited molecular state), causing it to drop to a lower energy level. The liberated energy transfers to the electromagnetic field, creating a new photon with a frequency, polarization, and direction of travel that are all identical to the photons of the incident wave. This is in contrast to spontaneous emission, which occurs at a characteristic rate for each of the atoms/oscillators in the upper energy state regardless of the external electromagnetic field.
Infrared spectroscopyInfrared spectroscopy (IR spectroscopy or vibrational spectroscopy) is the measurement of the interaction of infrared radiation with matter by absorption, emission, or reflection. It is used to study and identify chemical substances or functional groups in solid, liquid, or gaseous forms. It can be used to characterize new materials or identify and verify known and unknown samples. The method or technique of infrared spectroscopy is conducted with an instrument called an infrared spectrometer (or spectrophotometer) which produces an infrared spectrum.
System of linear equationsIn mathematics, a system of linear equations (or linear system) is a collection of one or more linear equations involving the same variables. For example, is a system of three equations in the three variables x, y, z. A solution to a linear system is an assignment of values to the variables such that all the equations are simultaneously satisfied. A solution to the system above is given by the ordered triple since it makes all three equations valid. The word "system" indicates that the equations should be considered collectively, rather than individually.
Electron microscopeAn electron microscope is a microscope that uses a beam of electrons as a source of illumination. They use electron optics that are analogous to the glass lenses of an optical light microscope. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.
Optical parametric amplifierAn optical parametric amplifier, abbreviated OPA, is a laser light source that emits light of variable wavelengths by an optical parametric amplification process. It is essentially the same as an optical parametric oscillator, but without the optical cavity (i.e., the light beams pass through the apparatus just once or twice, rather than many many times). Spontaneous parametric down conversion Optical parametric generation (OPG) (also called "optical parametric fluorescence", or "spontaneous parametric down conversion") often precedes optical parametric amplification.
