Publications related to An accurate PSF model with few parameters for axially shift-variant deconvolution

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

We report on studies of Thermobifida fusca cellulases Cel5A, Cel6B and Cel9A binding to pretreated wood particles using Confocal Laser Scanning Microscopy (CLSM). Hydro-thermal pretreated wood particles were immobilized on borosilicate substrates before fl ...

2011

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time seq ...

SPIE2010

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Cédric René Jean Vonesch

The subject of this thesis is image restoration, that is, deconvolution and denoising. Our work is motivated by applications in the rapidly expanding field of biological imaging and in particular fluorescence microscopy. Inverse problems in this area typic ...

EPFL2009

Super-resolution fluorescence microscopy based on physical models

François Aguet

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

EPFL2009

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

Theo Lasser, Iwan Märki

We demonstrate three-dimensional, nanometer-scale positioning precision of a single quantum-dot using single molecule interference and localization. We anticipate that our approach will lay the foundation for fluorescence microscopy with molecular resoluti ...

2009

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Accurate knowledge of an imaging system's point spread function (PSF) is crucial for successful deconvolution. For fluorescence microscopy, PSF estimations based on either theoretical models or experimental measurements are available. However, due to the ...

IEEE2008

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Emmanuel Guignet

Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacet ...

2007

A Fast Iterative Thresholding Algorithm for Wavelet-Regularized Deconvolution

Michaël Unser, Cédric René Jean Vonesch

We present an iterative deconvolution algorithm that minimizes a functional with a non-quadratic wavelet-domain regularization term. Our approach is to introduce subband-dependent parameters into the bound optimization framework of Daubechies et al.; it is ...

SPIE2007

An accurate PSF model with few parameters for axially shift-variant deconvolution

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Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Comparison of Deconvolution Software: A User Point of View—Part 2

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Super-resolution fluorescence microscopy based on physical models

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

A Fast Iterative Thresholding Algorithm for Wavelet-Regularized Deconvolution

Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy

Super-resolution fluorescence microscopy based on physical models

Comparison of Deconvolution Software: A User Point of View—Part 2

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

A Fast Iterative Thresholding Algorithm for Wavelet-Regularized Deconvolution

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution