Publications related to Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Pierre Gönczy, Suliana Manley, Debora Keller

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Public Library of Science2013

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Marc Chambon, Jonas Kühn, Etienne Shaffer, Jérôme Parent, Julien Mena

Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content ...

Mary Ann Liebert2013

Simulated biological cells for receptor counting in fluorescence imaging

Giovanni De Micheli, Sandro Carrara, Julien Michel Ghaye

Digital image processing and epi-fluorescence microscopy provide one of the main and basic tools for living biological cells analysis and studying. Developing, testing and comparing those image processing methods properly require the use of a controlled en ...

2012

Quantification of Sub-resolution Sized Targets in Cell Fluorescent Imaging

Giovanni De Micheli, Sandro Carrara, Julien Michel Ghaye

We introduce two methods for quantifying and evaluating the amount of surface receptors within a group of cells from fluorescence microscope images. First, the average fluorescence intensity method (AFIM), based on the fluores- cent pixels average intensit ...

2012

Accelerated autofocusing of off-axis holograms using critical sampling

Christian Depeursinge, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli, Stéphane Richard

In this Letter we propose a fast off-axis hologram autofocusing (AF) approach that is based on the redundant data elimination by the critical resampling of the contained complex field. Implementation of the proposed methodology enables the real-time AF wit ...

Optical Society of America2012

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Qiuling Xie

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maxim ...

Springer Netherlands2011

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

We demonstrate nanometer-level localization accuracy of a single fluorescent emitter in three dimensions. Our super resolution microscopy technique is based on spectral self-interference for axial localization and two-dimensional diffraction pattern analys ...

2009

Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

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Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Simulated biological cells for receptor counting in fluorescence imaging

Quantification of Sub-resolution Sized Targets in Cell Fluorescent Imaging

Accelerated autofocusing of off-axis holograms using critical sampling

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Quantification of Sub-resolution Sized Targets in Cell Fluorescent Imaging

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Simulated biological cells for receptor counting in fluorescence imaging

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Accelerated autofocusing of off-axis holograms using critical sampling

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy