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Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images

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Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing.

Saurabh Tomar

Polymerase chain reaction (PCR) has been the most significant driver in the field of nucleic acid testing (NAT) since its invention. Popularized as an abbreviation by the Covid-19 pandemic, PCR-based methods are the gold standard in the field of diagnostic ...

EPFL2023

Electrochemical sensors for the detection of SARS-CoV-2 virus

Somanath Jagannath, Neeraj Kumar

Coronavirus (COVID-19), a deadly pandemic has spread worldwide and created many global health issues. Though methods of its detection are being continuously developed for the early detection and monitoring of COVID-19, still there is need for more novel me ...

ELSEVIER SCIENCE SA2022

Using an unbiased high-throughput microRNA (miRNA)-silencing screen combined with functional readouts for mitochondrial oxidative capacity in C2C12 myocytes, we previously identified 19 miRNAs as putative regulators of skeletal muscle mitochondrial metabol ...

2020

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

Kai Johnsson, Lu Wang, Lin Xue

It is difficult to develop suitable fluorescent probes for live-cell nanoscopy, but a general strategy is now reported that can transform regular fluorophores into fluorogenic probes with excellent cell permeability and low unspecific background signals. U ...

NATURE PUBLISHING GROUP2020

Phase separation concentrates mitochondrial RNA granules. Here Rey et al., show that mitochondrial RNA granules (MRGs) behaviour is consistent with liquid-liquid phase separation (LLPS) and their fusion coincides with mitochondrial remodelling. Mitochondri ...

NATURE RESEARCH2020

Chemistry and Analysis of Nanoparticle-Modified Living Cells

Tanja Thomsen

Conventional therapeutics are often limited by their targeting ability, resulting in harmful and potential fatal side-effects for the patients. Recently, new strategies have been developed to improve target specificity of drugs in order to generate more ef ...

EPFL2020

A novel method is proposed in the scope of image segmentation that solves this problem by breaking it into two main blocks. The first block's functionality is a method to anticipate the color basis of each segment in segmented images. One of the challenges ...

IEEE2019

The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cell ...

Springer2019

Assessment of early response biomarkers in relation to long-term survival in patients with HER2-negative breast cancer receiving neoadjuvant chemotherapy plus bevacizumab: Results from the Phase II PROMIX trial

Eric Paquet

Pathologic complete response (pCR) is a predictor for favorable outcome after neoadjuvant treatment in early breast cancer. Modulation of gene expression may also provide early readouts of biological activity and prognosis, offering the possibility for tim ...

Wiley2018

Widefield High Frame Rate Single-Photon SPAD Imagers for SPIM-FCS

Edoardo Charbon, Claudio Bruschini, Andrei Ardelean, Samuel Burri

Photon-counting sensors based on standard complementary metal-oxide-semiconductor single-photon avalanche diodes (SPADs) represent an emerging class of imagers that enable the counting and/or timing of single photons at zero readout noise (better than high ...

2018